Semi Prep HPLC Column, HPS Silica, 5um, 120A. 10mm ID x 150mm Length. Cogent Brand. 1 EA.

Additional Info:

75001-SP150 Cogent HPS Silica™ Semi‑Prep HPLC Column is packed with high‑purity, unmodified silica, delivering consistent and reproducible performance for normal phase purification of polar and hydrophilic compounds.

Because the stationary phase is not chemically modified, it provides strong surface interactions with polar analytes, making it ideal for semi‑preparative purification of fats, lipids, and other hydrophilic compounds.

This proprietary HPS Silica™ has been widely adopted as a premium-value alternative to commonly used silica phases such as Inertsil® and Luna®, providing dependable selectivity and long-term reproducibility across laboratories.

The 5 µm particle size is well suited for semi‑prep workflows, allowing:

  • Higher flow rates

  • Lower system backpressure compared to smaller particles

  • Reliable performance under repeated purification cycles

150 mm Length – Enhanced Resolution for Purification

The 150 mm length provides increased resolving power for more challenging separations.

  • Higher resolution than 50 mm and 100 mm formats

  • Improved separation of closely related compounds

  • Increased peak capacity for complex mixtures

✅ Recommended for purification workflows requiring higher resolution and improved separation quality

10 mm ID – Semi‑Preparative Scale

  • Designed for semi‑preparative purification workflows

  • Increased sample loading capacity vs analytical columns

  • Suitable for analytical‑to‑prep scale‑up

  • Compatible with fraction collection systems

✅ Recommended for mid‑scale purification and process development

Flow Rate & System Requirements (10 mm ID, 5 µm)

⚠️ Requires semi‑preparative HPLC system capability

  • Typical flow rate range: ~4 – 12 mL/min

  • Low-to-moderate backpressure

  • Designed for higher flow purification

  • Compatible with preparative detectors and fraction collectors

✅ Important: A specialized HPLC system is required for columns of this size

Why Choose HPS Silica™ for Semi‑Prep

High‑Purity Unmodified Silica

  • Strong retention for polar compounds

  • Ideal for normal phase purification

USP L3 Compatibility

  • Suitable for compendial methods

  • Reliable alternative to traditional silica columns

Proven Performance

  • Widely used across HPLC labs

  • Consistent, reproducible results

Performance Advantages

Low Backpressure (5 µm Particles)

  • Enables higher flow rates

  • Compatible with prep-scale systems

Fast Equilibration

  • Reduces purification cycle times

Strong Polar Retention

  • Ideal for lipids and hydrophilic compounds

Typical Applications

  • Semi‑preparative purification of fats and lipids

  • Normal phase separations

  • Polar compound isolation

  • Method scale‑up from analytical HPLC

  • Pharmaceutical and chemical purification workflows

Important Usage Notes

  • Designed for normal phase chromatography conditions

  • Use appropriate non‑polar mobile phases

  • Requires semi‑preparative HPLC system capability

  • Ensure proper equilibration for reproducible results

Made‑to‑Order Columns & Pricing Information

This is a custom, made‑to‑order column.

  • Columns are manufactured specifically to order

  • Orders cannot be canceled once placed

  • Custom columns are non‑returnable

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